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Anti-TNFRSF1B Antibody EPR1653
Also for TNFRSF1B (NM_001066)
|A synthetic peptide corresponding to residues on the C-terminus in human TNFR2 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:250 - 1:500; FC: 1:10 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B)|
|CD120b; p75; p75TNFR; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80|
|TNFR2 is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of TNFR2 in protecting neurons from apoptosis by stimulating antioxidative pathways.|
Cytokine-cytokine receptor interaction
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Western blot - TNF Receptor II antibody [EPR1653]; All lanes : Anti-TNF Receptor II antibody [EPR1653] at 1/10000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : MCF7 cell lysate.Lane 3 : SW480 cell lysate.Lane 4 : THP1 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 48 kDa.Observed band size : 73 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TNF Receptor II antibody [EPR1653]; ab109322, at a 1/100 dilution, staining TNF Receptor II in paraffin embedded uterus tissue by Immunohistochemistry.
Flow Cytometry - Anti-TNF Receptor II antibody [EPR1653]; Overlay histogram showing HL60 cells stained with ab109322 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.