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Anti-TNFA Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA319290 Rabbit polyclonal TNF alpha antibody 1mg $325 3-7 Days
LC424626 TNF HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IHC(2)

OriGene Data

ImmunogenThe whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFa produced in E.coli.
Clone Name IsotypeIgG
Species Reactivityhuman Concentration1.0 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-a) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot using Anti-Human TNF-a (RABBIT) Antibody. Membrane blocked in 1% BSA-TBS-T 30 min RT, Rb-a-TNF alpha added at 1:1000 in 1% BSA-TBS-T o/n 4°C, DyLight 649 Gt-a-Rb 611-143-122 added at 1:20,000 in MB-070 30 min RT.
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Immunohistochemistry using polyclonal TNFa antibody showing staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum.  Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4°C.  HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.
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Fluorescent immunohistochemistry showing staining of human colon by anti-TNF alpha (formalin/PFA-fixed paraffin-embedded sections). Samples were formaldehyde-fixed, then blocked in 10% serum for 2 hours at 20°C. The primary antibody was diluted 1:100 and incubated with the sample for 2 hours at 20°C. Alexa Fluor® 680 goat polyclonal secondary antibody was used diluted 1:5000.
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