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Anti-TNF TRUEMAB Antibody Clone OTI1C1
TrueMAB Antibodies - Made against Authentic Protein Antigens
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|Full length human recombinant protein of human TNF(NP_000585) produced in HEK293T cell.|
|WB, IF, FC
||WB 1:4000, IF 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNF (RC206983, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNF.
Anti-TNF mouse monoclonal antibody (TA506513) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TNF(RC206983).
Immunofluorescent staining of HeLa cells using anti-TNF mouse monoclonal antibody (TA506513).
HEK293T cells transfected with either (RC206983) overexpress plasmid(Red) or empty vector control plasmid(blue) were immunostained by anti-TNF antibody(TA506513), and then analyzed by flow cytometry.(1:100)
Flow cytometric Analysis of Raw264.7 cells, using anti-TNF antibody(TA506513),(Red), compared to isotype control,(green), and negative control (PBS),(Blue)(1:100)