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Anti-TNF Antibody

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SKU Description Amount Price Availability*  
TA328455 Anti-Human TNF-a Rabbit Polyclonal Antibody 100ug $325 3-7 Days
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IHC(1)
ELISA(1)

OriGene Data

ImmunogenE.coli derived Recombinant Human TNF-α
Clone Name IsotypeIgG
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *ELISA, IHC Suggested DilutionsWB: 0.1-0.2ug/mL, ELISA: 0.25-2ug/mL, IHC: 0.25ug/mL - 1mg/mL
BufferA sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Purification Produced from sera of rabbits pre-immunized with highly pure recombinant Human TNF-alpha. Anti-Human TNF-alpha specific antibody was purified by affinity chromatography employing immobilized Human TNF-alpha matrix.
Note Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human TNF-alpha (0.5 ng/ml), a concentration of 0.08- 0.10 <=ug/ml of this antibody is required.

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

To detect Human TNF-alpha; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TNF-alpha; (TA328452) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-alpha;.
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To detect Human TNF-alpha; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TNF-alpha; (TA328452) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-alpha;.
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