TLR9 Mouse Monoclonal Antibody [Clone ID: 26C593.2]

CAT#: TA337047

Mouse Monoclonal TLR9 Antibody (26C593.2)

Product Images

Simple Western: TLR9 Antibody (26C593.2) TA337047 - Lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. Image using the Azide Free format of this antibody.

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  Western Blot: TLR9 Antibody (26C593.2) TA337047 - Analysis of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using this antibody at a dilution of 3 ug/ml.

Specifications

Product Data

• Clone Name: 26C593.2
• Applications: Block/Neutralize, CyTOF-ready, Dot, ELISA, FC, ICC/IF, IHC, IP, WB
• Recommended Dilution: Immunohistochemistry, Block/Neutralize, Knockdown Validated, Functional, In vitro assay, ELISA: 1:100 - 1:2000, Immunoprecipitation: 1:10 - 1:500, Flow (Intracellular): 1:10 - 1:1000, Dot Blot, Western Blot: 2-5 ug/ml, Immunohistochemistry-Paraffin: 5 ug/ml, Flow Cytometry, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Simple Western: 30 ug/ml, CyTOF-ready
• Reactivities: Human, Mouse, Rat, Canine, Equine, Primate
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: toll like receptor 9
• Database Link:
  NP_059138
  Entrez Gene 81897 MouseEntrez Gene 338457 RatEntrez Gene 54106 Human
  UniProt ID Q9NR96
• Synonyms: CD289
• Note: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In human PBMC, a ~120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases.

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Toll-like receptor signaling pathway