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Anti-TK1 PHOSPHO Antibody
|The antiserum was produced against synthesized phosphopeptide derived from human TK around the phosphorylation site of serine 13 (P-G-SP-P-S).|
||WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:5000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|TK (Phospho-Ser13) antibody detects endogenous levels of TK only when phosphorylated at serine 13.
|Homo sapiens thymidine kinase 1, soluble (TK1)|
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Western blot analysis of extracts from HeLa cells, treated with paclitaxel (1uM, 24hours), using TK (Phospho-Ser13) antibody.The lane on the right is treated with the synthesized peptide.Western blot analysis of extracts from JK cells (Lane 2) and A549 cells (Lane 3), using TK (Phospho-Ser13) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TK (Phospho-Ser13) antibody.The picture on the right is treated with the synthesized peptide.