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Home Antibody All anti-THEMIS antibodies

Anti-THEMIS Antibody EPR7353

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310895
  • Rabbit Monoclonal antibody against THEMIS
100ul 325 3-7 Days
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WB(1)
FC(1)
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Also for THEMIS (NM_001010923)
cDNA Clone shRNA/siRNA Primer Pair Protein Request Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human THEMIS was used as an immunogen.
Clone NameEPR7353 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; ICC: 1:50 - 1:100; FC: 1:10 - 1:100
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for IHC-P.

Reference Data

Target NameHomo sapiens thymocyte selection associated (THEMIS), transcript variant 2
Alternative NamebA325O24.3; bA325O24.4; C6orf190; C6orf207; GASP; SPOT; TSEPA
Database LinkNP_001010923
Entrez Gene 387357 Human
Entrez Gene 210757 Mouse
Entrez Gene 498985 Rat
FunctionTHEMIS plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. It functions through T-cell antigen receptor signaling, and it is necessary for proper lineage commitment and maturation of T-cells (1).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Themis antibody [EPR7353]; All lanes : Anti-Themis antibody [EPR7353] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : Human thymus lysates.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 73 kDa.
FC Image
Flow Cytometry - Anti-Themis antibody [EPR7353]; TA310895 at 1/10 dilution staining Themis in permeabilized Jurkat cells by Flow cytometry (red) compared with a rabbit IgG negative control (green).

 

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