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Home Antibody All anti-TEC antibodies

Anti-TEC Antibody Y398

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303712
  • Rabbit monoclonal antibody against Tec(Y398)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC418830) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
FC(1)
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Also for TEC (NM_003215)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the PH domain of human Tec was used as immunogen.
Clone NameY398 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:2000 - 1:5000; IHC-P: Use at an assay dependent concentration; ICC: 1:100 - 1:250; FC: 1:30 - 1:100; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified

Reference Data

Target NameHomo sapiens tec protein tyrosine kinase (TEC)
Alternative NamePSCTK4
Database LinkNP_003206
FunctionThe protein encoded by this gene belongs to the Tec family of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. Tec family kinases are involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. They are also key players in the regulation of the immune functions. Tec kinase is an integral component of T cell signaling and has a distinct role in T cell activation. This gene may be associated with myelodysplastic syndrome. [provided by RefSeq].
Related Pathway

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WB Image
Western blot - Tec antibody [Y398]; Anti-Tec antibody [Y398] at 1/10000 dilution + Jurkat cell lysate.Predicted band size : 73 kDa.Observed band size : 66 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Tec antibody [Y398]; TA303712, at a 1/50 dilution,staining human ovary carcinoma by immunohistochemistry, Paraffin embedded tissue.
FC Image
Flow Cytometry - Anti-Tec antibody [Y398]; Overlay histogram showing Jurkat cells stained with TA303712 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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