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Anti-TCF7L2 Antibody EP2033Y
Also for TCF7L2 (NM_030756)
|A synthetic peptide corresponding to residues near the N-terminus of human TCF-4 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:25000 - 1:100000; IP: 1:50; IHC-P: 1:100 - 1:250; FC: 1:50; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens transcription factor 7-like 2 (T-cell specific, HMG-box) (TCF7L2), transcript variant 2|
|TCF4 (HMG box transcription factor 4) is a member of the lymphoid enhancer factor family of transcription factors. Essential during development, along with beta catenin and Bcl 9, TCF4 participates in the Wnt signaling thru transactivation of downstreams genes (1). Commonly found in intestinal epithelium, TCF4 is required in the maintenance of crypt stem cell compartments of small intestines (2). Mutation in TCF4 has implicated in colorectal cancer and high expression of TCF4 variants has been linked in increased risk of type II diabetes (3). |
Wnt Signaling Pathway
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Western blot - TCF7L2 antibody [EP2033Y]; Anti-TCF7L2 antibody [EP2033Y] at 1/100000 dilution + Jurkat cell lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 68 kDa.Observed band size : 68 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCF7L2 antibody [EP2033Y]; ab76151 at 1/100 dilution staining T cell Transcription Factor 4 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - TCF7L2 antibody [EP2033Y]; ab76151 at 1/100 dilution staining T cell Transcription Factor 4 in HeLa cells by Immunofluorescence.
Flow Cytometry - Anti-TCF7L2 antibody [EP2033Y]; Overlay histogram showing Jurkat cells stained with ab76151 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.