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Anti-TCF7L2 Antibody EP2033Y
Also for TCF7L2 (NM_030756)
|A synthetic peptide corresponding to residues near the N-terminus of human TCF-4 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:25000 - 1:100000; IP: 1:50; IHC-P: 1:100 - 1:250; FC: 1:50; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Mouse, Rat
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Western blot - TCF7L2 antibody [EP2033Y]; Anti-TCF7L2 antibody [EP2033Y] at 1/100000 dilution + Jurkat cell lysate at 10 ug.Secondary.goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 68 kDa.Observed band size : 68 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCF7L2 antibody [EP2033Y]; TA301000 at 1/100 dilution staining T cell Transcription Factor 4 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - TCF7L2 antibody [EP2033Y]; TA301000 at 1/100 dilution staining T cell Transcription Factor 4 in HeLa cells by Immunofluorescence.
Flow Cytometry - Anti-TCF7L2 antibody [EP2033Y]; Overlay histogram showing Jurkat cells stained with TA301000 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.