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Anti-TBP Antibody EPR3826(2)
Also for TBP (NM_001172085)
|A synthetic peptide corresponding to residues in human TFIID was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; FC: 1:100 - 1:500
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens TATA box binding protein (TBP), transcript variant 2|
|GTF2D; GTF2D1; HDL4; SCA17; TFIID|
|Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. TFIID, a transcription factor, coordinates these activities and binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. It is TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. TFIID is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease (1).|
Wnt Signaling Pathway
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Western blot - Anti-TATA binding protein TBP antibody [EPR3826(2)]; All lanes : Anti-TATA binding protein TBP antibody [EPR3826(2)] at 1/1000 dilution.Lane 1 : PC12 cell lysate.Lane 2 : F9 cell lysate.Lane 3 : K562 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 38 kDa.Observed band size : 36 kDa .
Flow Cytometry - Anti-TATA binding protein TBP antibody [EPR3826(2)]; TA311083 at 1/50 dilution staining TATA binding protein TBP in permeabilized K562 cells by Flow cytometry (red) or a rabbit IgG negative control (green).