HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (RC212105, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.
HEK293T cells transfected with either RC212105 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TBC1D4 antibody(TA502695), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-TBC1D4 antibody(TA502695),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-TBC1D4 antibody(TA502695),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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