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Home Antibody All anti-IDE antibodies
KO-Validated

Anti-IDE TRUEMAB Antibody clone OTI4E5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA811776 IDE mouse monoclonal antibody,clone OTI4E5 100ul $325 In Stock
LC401545 IDE HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF811776 Carrier-free (BSA/glycerol-free) IDE mouse monoclonal antibody,clone OTI4E5 100ug $450 3-4 Weeks
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WB(2)

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 800-1019 of human IDE (NP_004960) produced in E.coli.
Clone Nameclone OTI4E5 IsotypeIgG2b
Species ReactivityHuman , Rat , Monkey Concentration1.00mg/ml
Guaranteed Application *WB Suggested Dilutions WB 1:500,
Predicted MW Explanation 117.8 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from cell culture supernatant by affinity chromatography

Reference Data

Target NameHomo sapiens insulin degrading enzyme (IDE), transcript variant 1
Alternative NameINSULYSIN; INSULYSIN
Database LinkNP_004960
FunctionThis gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009].
Related PathwayProteaseDruggable Genome Alzheimer's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and IDE-Knockout Hela cells (KO, Cat# LC810111) were separated by SDS-PAGE and immunoblotted with anti-IDE monoclonal antibody TA811776. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.(1:200)
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Western blot analysis of extracts (35ug) from 5 cell lines by using anti-IDE monoclonal antibody. (1:500)
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