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Home Antibody All anti-VRK1 antibodies
KO-Validated

Anti-VRK1 TRUEMAB Antibody Clone OTI2C9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA805595 VRK1 mouse monoclonal antibody, clone OTI2C9 (formerly 2C9) 100ul $325 In Stock
CF805595 Carrier-free (BSA/glycerol-free) VRK1 mouse monoclonal antibody, clone OTI2C9 (formerly 2C9) 100ug $450 3 Days
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OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 1-230 of human VRK1(NP_003375) produced in E.coli.
Clone NameClone OTI2C9 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB Suggested DilutionsWB 1:2000,
Predicted MW Explanation 45.3 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target Namevaccinia related kinase 1
Alternative NamePCH1|PCH1A
Database LinkNP_003375
Entrez Gene 7443 Human
FunctionThis gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008].
Related PathwayProtein KinaseDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VRK1 (RC210764, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VRK1.
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WB Image
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and VRK1-Knockout 293T cells (KO, Cat# LC810797) were separated by SDS-PAGE and immunoblotted with anti-VRK1 monoclonal antibody TA805595,(1:100). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
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