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Home Antibody All anti-DDOST antibodies

Anti-DDOST TRUEMAB Antibody Clone OTI2H2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA803618 DDOST mouse monoclonal antibody, clone OTI2H2 (formerly 2H2) 100ul $325 In Stock
LC417440 DDOST HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF803618 Carrier-free (BSA/glycerol-free) DDOST mouse monoclonal antibody, clone OTI2H2 (formerly 2H2) 100ug $450 3-4 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IHC(5)
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Also for DDOST (NM_005216)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 131-378 of human DDOST (NP_005207) produced in E.coli.
Clone NameClone OTI2H2 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB 1:500, IHC 1:150
Predicted MW Explanation 46.1 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic) (DDOST)
Alternative NameAGER1; CDG1R; OKSWcl45; OST; OST48; WBP1
Database LinkNP_005207
Entrez Gene 1650 Human
FunctionThis gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq, Jul 2008].
Related PathwayTransmembrane N-Glycan biosynthesisMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDOST (RC200672, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDOST.
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Western blot analysis of  A549 cell lysate (35ug) by using anti-DDOST monoclonal antibody. Dilution: 1:500
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803618)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803618)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803618)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803618)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DDOST mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803618)
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