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Home Antibody All anti-VIM antibodies

Anti-VIM TRUEMAB Antibody Clone OTI7F5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA801222 VIM (Vimentin) mouse monoclonal antibody, clone OTI7F5 (formerly 7F5) 100ul $325 In Stock
LC401165 VIM HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF801222 Carrier-free (BSA/glycerol-free) VIM mouse monoclonal antibody, clone OTI7F5 (formerly 7F5) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(2)

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 210-466 of human VIM (NP_003371) produced in E.coli.
Clone NameClone OTI7F5 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB Suggested DilutionsWB 1:200 - 1:1000
Predicted MW Explanation 53.5 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens vimentin (VIM)
Alternative NameCTRCT30; HEL113
Database LinkNP_003371
Entrez Gene 7431 Human
FunctionThis gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009].
Related PathwayES Cell Differentiation/IPS

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
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Western blot analysis of extracts (10ug) from 1 cell line by using anti-VIM monoclonal antibody at 1:200.
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