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Anti-PCNA TRUEMAB Antibody Clone OTI5F9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA800883 PCNA mouse monoclonal antibody, clone OTI5F9 (formerly 5F9) 100ul $325 In Stock
LC400929 PCNA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF800883 Carrier-free (BSA/glycerol-free) PCNA mouse monoclonal antibody, clone OTI5F9 (formerly 5F9) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)

OriGene Data

ImmunogenFull length human recombinant protein of human PCNA (NP_002583) produced in E.coli.
Clone NameClone OTI5F9 IsotypeIgG2b
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB Suggested Dilutions WB 1:2000,
Predicted MW Explanation 28.6 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens proliferating cell nuclear antigen (PCNA), transcript variant 1
Alternative NameATLD2
Database LinkNP_002583
Entrez Gene 5111 Human
FunctionThe protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008].
Related PathwayStem cell - PluripotencyDruggable Genome DNA replicationBase excision repairNucleotide excision repairMismatch repairCell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA (RC201741, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCNA.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PCNA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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