Home Antibody All anti-IDH1 antibodies
Anti-IDH1 TRUEMAB Antibody Clone OTI24A2
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for IDH1 (NM_005896)
|Synthetic peptide around the R132 region of the human IDH conjugated to KLH|
|Human , Dog , Rat , Monkey , Mouse
||WB 1:2000, IHC 1:150,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), transcript variant 1|
|HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD|
Entrez Gene 3417 Human
Entrez Gene 15926 Mouse
Entrez Gene 24479 Rat
Entrez Gene 710019 Monkey
Entrez Gene 478889 Dog
|Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq, Jul 2008].|
| Citrate cycle (TCA cycle)Glutathione metabolismMetabolic pathways|
* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA
) and IDH1-Knockout hela cells (KO,Cat# LC810112
) were separated by SDS-PAGE and immunoblotted with anti-IDH1 monoclonal antibody TA800426
. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494
) as a loading control.(1:500)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDH1 (RC210582, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDH1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were either not tranfected (left lane "293T") or transfected with pCMV6-ENTRY IDH1(wild type-SKU# RC210582, middle lane "WT") or pCMV6-ENTRY IDH1 mutated (R132H mutation-SKU# RC400096, right lane "R132H") cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (10 ug per lane) were separated by SDS-PAGE and immunoblotted with TA800426 (1:500) and then goat anti-mouse IgG-HRP (1:2000).
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-IDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800426)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-IDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800426)