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Home Antibody All anti-APEX1 antibodies
KO-Validated

Anti-APEX1 TRUEMAB Antibody Clone OTI1H9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA800330 APEX1 mouse monoclonal antibody, clone OTI1H9 (formerly 1H9) 100ul $325 In Stock
LC400618 APEX1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF800330 Carrier-free (BSA/glycerol-free) APEX1 mouse monoclonal antibody, clone OTI1H9 (formerly 1H9) 100ug $450 3 Days
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WB(3)
IHC(6)
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Also for APEX1 (NM_001641)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 1-242 of human APEX1 (NP_001632) produced in E.coli.
Clone NameClone OTI1H9 IsotypeIgG1
Species ReactivityHuman , Dog , Monkey Concentration1 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB 1:2000, IHC 1:150,
Predicted MW Explanation 35.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens apurinic/apyrimidinic endodeoxyribonuclease 1 (APEX1), transcript variant 1
Alternative NameAPE; APE1; APEN; APEX; APX; HAP1; REF1
Database LinkNP_001632
Entrez Gene 328 Human
Entrez Gene 702757 Monkey
Entrez Gene 482558 Dog
FunctionApurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008].
Related PathwayStem cell - PluripotencyTranscription FactorsDruggable Genome Base excision repair

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APEX1 (RC201732, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APEX1.
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WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-APEX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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WB Image
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and APEX1-Knockout 293T cells (KO, Cat# LC811481) were separated by SDS-PAGE and immunoblotted with anti-APEX1 monoclonal antibody TA800330,(1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
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IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800330)
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IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800330)
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IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800330)
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IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800330)
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IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800330)
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IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800330)
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