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Anti-MRPS27 TRUEMAB Antibody Clone OTI3B9
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for MRPS27 (NM_015084)
|Human recombinant protein fragment corresponding to amino acids 151-414 of human MRPS27 (NP_055899) produced in E.coli.|
|WB, IHC, FC
|| WB 1:500~2000, IHC 1:150, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 2|
Entrez Gene 23107 Human
|Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). [provided by RefSeq]. COMPLETENESS: complete on the 3' end. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (RC209478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MRPS27 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800199)
HEK293T cells transfected with either RC209478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MRPS27 antibody(TA800199), and then analyzed by flow cytometry.