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Home Antibody All anti-MRPS27 antibodies

Anti-MRPS27 TRUEMAB Antibody Clone OTI3D9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA800198 MRPS27 mouse monoclonal antibody, clone OTI3D9 (formerly 3D9) 100ul $379 In Stock
LC402416 MRPS27 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF800198 Carrier-free (BSA/glycerol-free) MRPS27 mouse monoclonal antibody, clone OTI3D9 (formerly 3D9) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IHC(1)
FC(1)
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Also for MRPS27 (NM_015084)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 151-414 of human MRPS27 (NP_055899) produced in E.coli.
Clone NameClone OTI3D9 IsotypeIgG2b
Species ReactivityHuman , Monkey Concentration1 mg/ml
Guaranteed Application *WB, IHC, FC Suggested Dilutions WB 1:500~2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 47.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 2
Alternative NameMRP-S27; S27mt
Database LinkNP_055899
Entrez Gene 23107 Human
Entrez Gene 701585 Monkey
FunctionMammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). [provided by RefSeq, Jul 2008].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (RC209478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MRPS27 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800198)
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HEK293T cells transfected with either RC209478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MRPS27 antibody(TA800198), and then analyzed by flow cytometry.
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