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Anti-NUB1 TRUEMAB Antibody Clone 4H2
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for NUB1 (NM_016118)
|Human recombinant protein fragment corresponding to amino acids 1-326 of human NUB1 (NP_057202) produced in E.coli.|
|Human, Dog, Rat, Monkey
|WB, IHC, IF, FC
||WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens negative regulator of ubiquitin-like proteins 1 (NUB1), transcript variant 2|
|BS4; NUB1L; NYREN18|
|NUB1 interacts with and negatively regulates NEDD8 (MIM 603171), a ubiquitin-like protein that covalently conjugates to cullin (see MIM 603134) family members.[supplied by OMIM]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUB1 (RC208098, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUB1.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-NUB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA800188)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA800188)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA800188)
Anti-NUB1 mouse monoclonal antibody (TA800188) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUB1(RC208098).
HEK293T cells transfected with either RC208098 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NUB1 antibody(TA800188), and then analyzed by flow cytometry.