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Anti-SKIL TrueMAB Antibody Clone 1H2
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for SKIL (NM_005414)
|Human recombinant protein fragment corresponding to amino acids 307-684 of human SKIL (NP_005405) produced in E.coli.|
||0.5~1.0 mg/ml (Lot Dependent)
|| WB 1:500~1000,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens SKI-like oncogene (SKIL), transcript variant 1|
|SNO; SnoA; SnoI; SnoN|
|The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]. |
TGF Beta Signaling Pathway
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SKIL (RC209251, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SKIL.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SKIL monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).