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Home Antibody All anti-SHH antibodies
UltraMAB

Anti-SHH GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA590806
  • Rabbit Polyclonal Sonic Hedgehog Antibody
100ug 325 In Stock
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(1)
IHC(1)
IF(1)
ELISA(1)
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Also for SHH (NM_000193)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the Middle Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman Concentration1mg/ml
Guaranteed Application *WB, ELISA, IHC, IF Suggested DilutionsWB: 1:500; ELISA: 1:100-1:2000; IHC: 1:350; IF: 1:87
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens sonic hedgehog (SHH)
Alternative NameHHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS
Database LinkNP_000184
Entrez Gene 6469 Human
FunctionSonic Hedgehog Protein (SHH, VHH-1) belongs to hedgehog protein family which includes Indian Hh, and Desert Hh. Hh family is involved in the cell fate and patterning during embryonic development, homeostasis, and adult tissue renewal (1). Similar to other member in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene (2). Precursor SHH is autocatlytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in the signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo (3). A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which developing forebrain fails to separate into right and left hemispheres, and ocular coloboma (4).
Related PathwaySecreted ProteinES Cell Differentiation/IPSTransmembraneDruggable Genome Hedgehog signaling pathwayPathways in cancerBasal cell carcinoma

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA Image
ELISA: Sonic Hedgehog Antibody
IHC Image
Immunohistochemical staining of human kidney shows cytoplasmic and membranous positivity in cells of renal tubules.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
IF Image
Immunofluorescent staining of human cell line A-431 shows no positivity.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

 

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