Home Antibody All anti-SHH antibodies
Anti-SHH GAT Antibody Polyclonal
Generated by Genomic Immunization
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|DNA immunization. This antibody is specific for the Middle Region of the target protein.|
|WB, ELISA, IHC, IF
||WB: 1:500; ELISA: 1:100-1:2000; IHC: 1:350; IF: 1:87
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens sonic hedgehog (SHH), transcript variant 1|
|HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS|
Entrez Gene 6469 Human
|Sonic Hedgehog Protein (SHH, VHH-1) belongs to hedgehog protein family which includes Indian Hh, and Desert Hh. Hh family is involved in the cell fate and patterning during embryonic development, homeostasis, and adult tissue renewal (1). Similar to other member in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene (2). Precursor SHH is autocatlytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in the signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo (3). A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which developing forebrain fails to separate into right and left hemispheres, and ocular coloboma (4).|
|Secreted ProteinES Cell Differentiation/IPSTransmembraneDruggable Genome Hedgehog signaling pathwayPathways in cancerBasal cell carcinoma|
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Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA: Sonic Hedgehog Antibody
Immunohistochemical staining of human kidney shows cytoplasmic and membranous positivity in cells of renal tubules.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line A-431 shows no positivity.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.