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Anti-IGFBP2 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for IGFBP2 (NM_000597)
|DNA immunization. This antibody is specific for the Middle Region of the target protein.|
|Human , Monkey
|WB, ELISA, IHC
|| WB 1:5000~20000, IHC 1:150, ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens insulin-like growth factor binding protein 2, 36kDa (IGFBP2)|
|IGFBP2 is a member of the ISGBP family which bind various IGFs. IGFBP2 is over expressed in a wide spectrum of other cancers, including glioma, prostate cancer, synovial sarcoma, neuroblastoma, colon cancer, adrenocortical cancer, lung cancer, Wilms' tumor, and hepatoblastoma. The over expression of IGFBP2 also correlates with the aggressiveness of some tumors. IGFBP2 activates the expression of matrix metalloprotease 2, which contributes to cell invasiveness.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGFBP2 (RC202573, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGFBP2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IGFBP2 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-IGFBP2 Rabbit Polyclonal antibody. (TA590786)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IGFBP2 Rabbit Polyclonal antibody. (TA590786)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-IGFBP2 Rabbit Polyclonal antibody. (TA590786)