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Anti-EGF GAT Antibody Polyclonal
UltraMAB Antibodies - Validated with 10K Protein Chip
|DNA immunization. This antibody is specific for the C Terminus Region of the target protein.|
||Dot Blot 1:100-1:2000; ELISA: 1:100-1:2000; IHC: 1:10-1:2000; IHC-P 1:250-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody. (Protein A or G Sepharose)
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens epidermal growth factor (EGF), transcript variant 1|
|Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.|
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
TGF Beta Signaling Pathway
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Dot Blot: EGF Antibody - The anti-EGF antibody (left quadruplicate spots at each panel) or control antibody (right quadruplicate spots at each panel) were printed on membranes at 10 ng/spot. The membranes were incubated with biotinylated cell lysate (50 ug per membrane), followed by probing with streptavidin-HRP and CCD camera image acquisition. (a), Cell lysate from Jurkat cells; (b), Cell lysate from 293T cells. Data courtesy of a SDI customer.
Dot Blot: EGF Antibody - Protein samples (left spot: 293T cell lysate; right spot: Jurkat cell lysate) were printed on membranes at 40 ng/spot. The membrane was incubated with anti-EGF antibody (1:4000 dilution) and then probed anti-rabbit IgG-HRP. Signals were acquired with a CCD camera. Data courtesy of a SDI customer.