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Home Antibody All anti-PCK1 antibodies
UltraMAB

Anti-PCK1 GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA590679
  • Rabbit Polyclonal PCK1 Antibody
100ug 325 In Stock
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WB(3)
IHC(2)
ELISA(1)
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Also for PCK1 (NM_002591)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the Middle Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Rat , Monkey , Mouse Concentration1.05mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:5000~20000, IHC 1:150, ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens phosphoenolpyruvate carboxykinase 1 (soluble) (PCK1)
Alternative NamePEPCK-C; PEPCK1; PEPCKC
Database LinkNP_002582
FunctionMajor sites of expression of PEP Carboxylase are liver, kidney and adipocytes. PEP carboxylase, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein has also been characterized.
Related Pathway
Adipogenesis

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WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCK1 (RC204758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCK1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PCK1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
WB Image
Western Blot: PCK1 Antibody - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight: 69 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.
ELISA Image
ELISA: PCK1 Antibody
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PCK1 Rabbit Polyclonal antibody. (TA590679)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PCK1 Rabbit Polyclonal antibody. (TA590679)

 

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