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Anti-PCK1 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for PCK1 (NM_002591)
|DNA immunization. This antibody is specific for the Middle Region of the target protein.|
|Human , Rat , Monkey , Mouse
|WB, ELISA, IHC
||WB 1:5000~20000, IHC 1:150, ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCK1 (RC204758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCK1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PCK1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western Blot: PCK1 Antibody - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight: 69 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PCK1 Rabbit Polyclonal antibody. (TA590679)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PCK1 Rabbit Polyclonal antibody. (TA590679)