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Anti-E2F1 GAT Antibody Polyclonal
UltraMAB Antibodies - Validated with 10K Protein Chip
Also for E2F1 (NM_005225)
|DNA immunization. This antibody is specific for the C Terminus Region of the target protein.|
|ELISA, IHC, IF
||ELISA: 1:100-1:2000; IHC: 1:250; IF: 1:62
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody. (Protein A or G Sepharose)
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens E2F transcription factor 1 (E2F1)|
|E2F-1; RBAP1; RBBP3; RBP3|
|E2F's are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F1 overexpression can also drive cells into apoptosis.|
Senescence and Autophagy
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Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus & nucleoli.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.