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Anti-HSPA5 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for HSPA5 (NM_005347)
|DNA immunization. This antibody was made against a protein fragment from the C Terminus Region|
|Human , Dog , Rat , Monkey , Mouse
|WB, IHC, IF
|| WB 1:5000~20000, IHC 1:150, IF 1:500,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA5 (RC205859, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA5.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA5 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HSPA5 Rabbit Polyclonal antibody. (TA590608)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HSPA5 Rabbit Polyclonal antibody. (TA590608)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HSPA5 Rabbit Polyclonal antibody. (TA590608)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HSPA5 Rabbit Polyclonal antibody. (TA590608)
Immunofluorescent staining of HeLa cells using anti-HSPA5 rabbit polyclonal antibody (TA590608).