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Anti-LHX1 GAT Antibody Polyclonal
UltraMAB Antibodies - Validated with 10K Protein Chip
Also for LHX1 (NM_005568)
|DNA immunization. This antibody is specific for the C Terminus Region of the target protein.|
||WB: 1:5000-1:20000; ELISA: 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody. (Protein A or G Sepharose)
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens LIM homeobox 1 (LHX1)|
|This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator and be involved in control of differentiation and development of neural and lymphoid cells. A similar protein in mice is an essential regulator of the vertebrate head organizer.|
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Western Blot: LIM1 Antibody - Western Blot was performed using affinity purified SEQer LHX1 antibody, aa(267-366) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer LHX1 antibody, aa(267-366) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.