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UltraMAB

Anti-LHX1 GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA590598 Rabbit Polyclonal LIM1 Antibody 100ug In Stock
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WB(3)
IHC(6)
ELISA(1)

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the C Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Monkey , Mouse Concentration1mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens LIM homeobox 1 (LHX1)
Alternative NameLIM-1; LIM1
Database LinkNP_005559
Entrez Gene 3975 Human
Entrez Gene 16869 Mouse
FunctionThis gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator and be involved in control of differentiation and development of neural and lymphoid cells. A similar protein in mice is an essential regulator of the vertebrate head organizer.
Related PathwayES Cell Differentiation/IPSTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LHX1 (RC210977, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LHX1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LHX1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Western Blot: LIM1 Antibody - Western Blot was performed using affinity purified SEQer LHX1 antibody, aa(267-366) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer LHX1 antibody, aa(267-366) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
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ELISA: LIM1 Antibody
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-LHX1 Rabbit Polyclonal antibody. (TA590598)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LHX1 Rabbit Polyclonal antibody. (TA590598)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-LHX1 Rabbit Polyclonal antibody. (TA590598)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-LHX1 Rabbit Polyclonal antibody. (TA590598)
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-LHX1 Rabbit Polyclonal antibody. (TA590598)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-LHX1 Rabbit Polyclonal antibody. (TA590598)
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