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Anti-ROPN1 GAT Antibody Polyclonal
UltraMAB Antibodies - Validated with 10K Protein Chip
Also for ROPN1 (NM_017578)
|DNA immunization. This antibody is specific for the N Terminus Region of the target protein.|
|WB, ELISA, IHC
||WB: 0.5-1.5 ug/ml; ELISA: 1:100-1:2000; IHC: 1:10-1:2000; IHC-P 1:10-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody. (Protein A or G Sepharose)
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens rhophilin associated tail protein 1 (ROPN1)|
|CT91; ODF6; RHPNAP1; ROPN1A; ropporin|
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Western Blot: ROPN1 Antibody - Western Blot was performed using affinity purified SEQer ROPN1 antibody, aa(1-99) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer ROPN1 antibody, aa(1-99) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
Immunohistochemistry: ROPN1 Antibody - Normal tissues showed moderate to strong cytoplasmic staining. Additional membranous positivity was sometimes seen. Testis, gastro-intestinal tract, parathyroid gland, placenta, spleen and myocytes displayed strong cytoplasmic immunoreactivity. A majority of cancers displayed moderate cytoplasmic staining. Colo-rectal, renal, stomach and liver malignancies exhibited moderate to strong positivity. Gliomas, lymphomas, melanomas, skin and lung cancers were weakly to moderately stained. Image and statement courtesy of the Human Protein Atlas (HPA).