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Home Antibody All anti-AMBRA1 antibodies
UltraMAB

Anti-AMBRA1 GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA590501 Rabbit Polyclonal AMBRA1 Antibody 100ug $325 In Stock
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WB(2)
IP(1)
IHC(7)
IF(2)
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Also for AMBRA1 (NM_017749)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenDNA immunization. This antibody was made against a protein fragment from the C Terminus Region of AMBRA1.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman Concentration1.05mg/ml
Guaranteed Application *WB, IHC, IF, IP Suggested DilutionsWB 1:20000, IHC 1:150, IF 1:500,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant 2
Alternative NameDCAF3; WDR94
Database LinkNP_060219
Entrez Gene 55626 Human
FunctionAMBRA1 regulates autophagy and development of the nervous system. It is a large, previously unknown protein bearing a WD40 domain at its amino terminus, regulates autophagy and has a crucial role in embryogenesis. AMBRA1 is a positive regulator of the Becn1-dependent programme of autophagy, as revealed by its overexpression and by RNA interference experiments invitro. Notably, AMBRA1 functional deficiency in mouse embryos leads to severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic cell death.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMBRA1 (RC206255, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMBRA1.
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WB Image
Western Blot: AMBRA1 Antibody - Western blot analysis of AMBRA1 on brain extracts using TA590501 showing a band at approximately 132kDa
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IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AMBRA1 rabbit polyclonal antibody. (TA590501)
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IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-AMBRA1 rabbit polyclonal antibody. (TA590501)
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IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-AMBRA1 rabbit polyclonal antibody. (TA590501)
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IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-AMBRA1 rabbit polyclonal antibody. (TA590501)
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IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-AMBRA1 rabbit polyclonal antibody. (TA590501)
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IHC Image
Immunohistochemistry: AMBRA1 Antibody - Immunohistochemical analysis of AMBRA1 on human cerebral cortex tissue
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IHC Image
Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
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IF Image
Immunofluorescent staining of HeLa cells using anti-AMBRA1 rabbit polyclonal antibody (TA590501).
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IF Image
Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
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IP Image
Immunoprecipitation: AMBRA1 Antibody - IP analysis of AMBRA1 in various cell lysates.
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