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Anti-CASP7 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for CASP7 (NM_033338)
|DNA immunization. This antibody is specific for the N Terminus Region of the target protein.|
|Human , Monkey , Mouse
|WB, ELISA, IHC
|| WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens caspase 7, apoptosis-related cysteine peptidase (CASP7), transcript variant d|
|CASP-7; CMH-1; ICE-LAP3; LICE2; MCH3|
|Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase-8 and Caspase-9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse.|
MAPK signaling pathway
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CASP7 (RC221589, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CASP7.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CASP7 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
ELISA: Caspase 7 Antibody - Affinity Purified
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CASP7 rabbit polyclonal antibody. (TA590426)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-CASP7 rabbit polyclonal antibody. (TA590426)