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Home Antibody All anti-DCLK3 antibodies
UltraMAB

Anti-DCLK3 GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA590425
  • Rabbit Polyclonal DCLK3 Antibody
100ug $325 In Stock
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WB(2)
IHC(1)
ELISA(1)

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the N Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman Concentration1.2mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested DilutionsWB: 1:5000-1:20000; ELISA: 1:100-1:2000; IHC: 1:10-1:2000; IHC-P 1:10-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens doublecortin-like kinase 3 (DCLK3)
Alternative NameCLR; DCAMKL3; DCDC3C; DCK3
Database LinkNP_208382
Entrez Gene 85443 Human
Function
Related PathwayProtein KinaseDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western Blot: DCLK3 Antibody - Western Blot was performed using affinity purified SEQer DCAMKL3 antibody, aa(138-237) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer DCAMKL3 antibody, aa(138-237) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
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Western Blot: DCLK3 Antibody - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight: 74 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.
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ELISA: DCLK3 Antibody
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Immunohistochemistry: DCLK3 Antibody - Normal tissues generally displayed weak to moderate cytoplasmic and occasional nuclear positivity. Ciliated cells in airways and fallopian tube exhibited distinct membranous staining. Cells for example in liver, prostate and spleen were negative. Colo-rectal and endometrial cancers exhibited some moderately stained cases while malignant gliomas, ovarian and stomach cancers showed several weakly immunoreactive samples. Remaining malignant tissues were mainly negative. Image and statement courtesy of the Human Protein Atlas (HPA).
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