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Anti-STOML3 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for STOML3 (NM_145286)
|DNA immunization. This antibody is specific for the C Terminus Region of the target protein.|
|WB, ELISA, IHC
|| WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens stomatin (EPB72)-like 3 (STOML3), transcript variant 1|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STOML3 (RC206682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STOML3.
Western Blot: STOML3 Antibody - Western Blot was performed using affinity purified SEQer STOML3 antibody, aa(192-291) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer STOML3 antibody, aa(192-291) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
Western Blot: STOML3 Antibody - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight: 32 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-STOML3 Rabbit Polyclonal antibody. (TA590398)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-STOML3 Rabbit Polyclonal antibody. (TA590398)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-STOML3 Rabbit Polyclonal antibody. (TA590398)
Immunohistochemistry: STOML3 Antibody - Cells in lymphoid follicles were negative. Endothelial cells were distinctly stained. Strong membranous positivity was observed in fallopian tube, hepatocytes and gallbladder. Among malignancies, most tissues displayed moderate cytoplasmic staining. Distinct additional membranous positivity was observed in several adenocarcinomas of colon, breast, cervix, liver, ovary and also in melanomas. Image and statement courtesy of the Human Protein Atlas (HPA).