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Anti-STOML3 GAT Antibody Polyclonal
Generated by Genomic Immunization
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Also for STOML3 (NM_145286)
|DNA immunization. This antibody is specific for the C Terminus Region of the target protein.|
|WB, ELISA, IHC
|| WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STOML3 (RC206682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STOML3.
Western Blot: STOML3 Antibody - Western Blot was performed using affinity purified SEQer STOML3 antibody, aa(192-291) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer STOML3 antibody, aa(192-291) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
Western Blot: STOML3 Antibody - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight: 32 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-STOML3 Rabbit Polyclonal antibody. (TA590398)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-STOML3 Rabbit Polyclonal antibody. (TA590398)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-STOML3 Rabbit Polyclonal antibody. (TA590398)
Immunohistochemical staining of human liver shows membranous positivity in hepatocytes. Antibody dilution: 1:150. Image and statement courtesy of the Human Protein Atlas (HPA).