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Home Antibody All anti-STOML3 antibodies
UltraMAB

Anti-STOML3 GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA590398 Rabbit Polyclonal STOML3 Antibody 100ug $325 In Stock
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WB(3)
IHC(4)
ELISA(1)

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the C Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman Concentration0.9mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens stomatin (EPB72)-like 3 (STOML3), transcript variant 1
Alternative NameEpb7.2l; SRO
Database LinkNP_660329
Entrez Gene 161003 Human
Function
Related PathwayTransmembrane

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STOML3 (RC206682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STOML3.
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Western Blot: STOML3 Antibody - Western Blot was performed using affinity purified SEQer STOML3 antibody, aa(192-291) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer STOML3 antibody, aa(192-291) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
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Western Blot: STOML3 Antibody - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight: 32 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.
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ELISA: STOML3 Antibody
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Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-STOML3 Rabbit Polyclonal antibody. (TA590398)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-STOML3 Rabbit Polyclonal antibody. (TA590398)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-STOML3 Rabbit Polyclonal antibody. (TA590398)
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Immunohistochemical staining of human liver shows membranous positivity in hepatocytes. Antibody dilution: 1:150. Image and statement courtesy of the Human Protein Atlas (HPA).
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