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Home Antibody All anti-CHEK2 antibodies
UltraMAB

Anti-CHEK2 GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA590397
  • Rabbit Polyclonal Chk2 Antibody
100ug 325 In Stock
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WB(1)
IHC(7)
ELISA(1)

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the N Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman Concentration0.87mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens checkpoint kinase 2 (CHEK2), transcript variant 2
Alternative NameCDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53
Database LinkNP_665861
FunctionCHK2 is a serine/threonine kinase involved in the control of cell cycle checkpoints, and may also participate in transduction of the DNA damage and replicational stress signals. CHK2 is the mammalian ortholog of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases. The amino-terminal domain of CHK2 contains a series of seven serine and threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases. Indeed, after DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain. CHK2 inhibits CDC25C phosphatase by phosphorylating it on Ser-216, preventing the entry into mitosis. This kinase may have a role in meiosis as well. Kinase activity is up regulated by autophosphorylation and the protein is rapidly phosphorylated in response to DNA damage and to replication block. Defects in CHEK2 are associated with Li-Fraumeni syndrome (LFS); a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. Defects in CHEK2 are also found in some patients with prostate cancer (CaP) or osteosarcoma (OSRC). Substantial amino acid variants exist in some cancer tissues. Numerous truncated splice variants exist for this protein.
Related Pathway
Cell cycle

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WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC215909, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
ELISA Image
ELISA: Chk2 Antibody
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CHEK2 Rabbit Polyclonal antibody. (TA590397)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CHEK2 Rabbit Polyclonal antibody. (TA590397)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CHEK2 Rabbit Polyclonal antibody. (TA590397)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CHEK2 Rabbit Polyclonal antibody. (TA590397)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CHEK2 Rabbit Polyclonal antibody. (TA590397)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-CHEK2 Rabbit Polyclonal antibody. (TA590397)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CHEK2 Rabbit Polyclonal antibody. (TA590397)

 

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