Home Antibody All anti-RASGRF1 antibodies
Anti-RASGRF1 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for RASGRF1 (NM_153815)
|DNA immunization. This antibody is specific for the C Terminus Region of the target protein.|
||WB: 1:5000-1:20000; ELISA: 1:100-1:2000; IHC: 1:10-1:2000; IHC-P 1:250-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 2|
|CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55; PP13187; ras-GRF1|
|A critical step in signal transduction responses to stimulation of cell surfacereceptors by their ligands involves the accumulation of Ras proteins in theiractive GTP-bound state. To reach their active GTP-bound state, Ras proteinsmust first release bound GDP, a rate limiting step mediated by a guaninenucleotide releasing factor (GRF). The mammalian Ras p21 GRF protein hasbeen designated Ras-GRF1 p140. Ras-GRF1 accelerates release of GDP fromH- and N-Ras p21 protein in vitro, but not from the related Ral A or Cdc42HsGTP-binding proteins. Of interest, a region mapping within the amino terminaldomain of Ras-GRF1 is similar to both the human breakpoint cluster protein,Bcr, and the Dbl proto-oncogene product, a guanine nucleotide-releasingfactor for Cdc42Hs. Ras-GRF2 p135 has also been identified. Ras-GRF2p135 is highly homologous to Ras-GRF1 p140 except in the region betweenthe REM and CDC25 domains and appears to function similarly to Ras-GRF1p140.|
MAPK signaling pathway
Signaling by GPCR
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