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Anti-RFC4 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for RFC4 (NM_181573)
|DNA immunization. This antibody is specific for the C Terminus Region of the target protein.|
|WB, ELISA, IHC
||WB 1:20000, IHC 1:150, ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 2|
|The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4 (RC211392, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFC4.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RFC4 rabbit polyclonal antibody. (TA590357)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RFC4 rabbit polyclonal antibody. (TA590357)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-RFC4 rabbit polyclonal antibody. (TA590357)