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Home Antibody All anti-CASP3 antibodies
UltraMAB

Anti-CASP3 GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA590291 Rabbit Polyclonal Caspase 3 Antibody 100ug $325 In Stock
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WB(2)
IHC(4)
ELISA(1)
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Also for CASP3 (NM_032991)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Assay

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the Middle Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration1.3mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta
Alternative NameCPP32; CPP32B; SCA-1
Database LinkNP_116786
Entrez Gene 836 Human
Entrez Gene 12367 Mouse
Entrez Gene 25402 Rat
Entrez Gene 694473 Monkey
Entrez Gene 403567 Dog
FunctionCaspase 3 is ubiquitously expressed and like other caspases is synthesized as an inactive (32kDa) proenzyme. Upon activation, Caspase 3 is cleaved at Asp28-Ser29 and Asp175-Ser176 thereby generating two subunits of 17kDa and 12kDa, respectively. Recent studies have implicated that Caspase 3 is associated with the induction of apoptosis. Activation of Caspase 3 occurs in response to variety of apoptotic inducers including Fas mediated apoptosis.
Related PathwayES Cell Differentiation/IPSProteaseDruggable Genome MAPK signaling pathwayp53 signaling pathwayApoptosisNatural killer cell mediated cytotoxicityAlzheimer's diseaseParkinson's diseaseMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CASP3 (RC204444, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CASP3.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CASP3 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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ELISA: Caspase 3 Antibody
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Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-CASP3 Rabbit Polyclonal antibody. (TA590291)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-CASP3 Rabbit Polyclonal antibody. (TA590291)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CASP3 Rabbit Polyclonal antibody. (TA590291)
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CASP3 Rabbit Polyclonal antibody. (TA590291)
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