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Anti-CASP3 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for CASP3 (NM_032991)
|DNA immunization. This antibody is specific for the Middle Region of the target protein.|
|Human , Dog , Rat , Monkey , Mouse
|WB, ELISA, IHC
|| WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta|
|CPP32; CPP32B; SCA-1|
|Caspase 3 is ubiquitously expressed and like other caspases is synthesized as an inactive (32kDa) proenzyme. Upon activation, Caspase 3 is cleaved at Asp28-Ser29 and Asp175-Ser176 thereby generating two subunits of 17kDa and 12kDa, respectively. Recent studies have implicated that Caspase 3 is associated with the induction of apoptosis. Activation of Caspase 3 occurs in response to variety of apoptotic inducers including Fas mediated apoptosis.|
MAPK signaling pathway
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CASP3 (RC204444, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CASP3.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CASP3 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-CASP3 Rabbit Polyclonal antibody. (TA590291)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-CASP3 Rabbit Polyclonal antibody. (TA590291)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CASP3 Rabbit Polyclonal antibody. (TA590291)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CASP3 Rabbit Polyclonal antibody. (TA590291)