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Anti-ABCF2 GAT Antibody Polyclonal
Generated by Genomic Immunization
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Also for ABCF2 (NM_007189)
|DNA immunization. This antibody is specific for the N Terminus Region of the target protein.|
|Human , Rat
|WB, ELISA, IHC, IF
|| WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens ATP-binding cassette, sub-family F (GCN20), member 2 (ABCF2), transcript variant 1|
|ABC28; EST133090; HUSSY-18; HUSSY18|
Entrez Gene 10061 Human
Entrez Gene 311959 Rat
|ABCF2 is a member of the ABC Transporter superfamily and of the GCN20 subfamily. As a member of the ABC Transporter family, it functions to transport various molecules across extra- and intracellular membranes.|
|Druggable Genome |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCF2 (RC200323, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABCF2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ABCF2 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ABCF2 rabbit polyclonal antibody. (TA590235)
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ABCF2 rabbit polyclonal antibody. (TA590235)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-ABCF2 rabbit polyclonal antibody. (TA590235)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-ABCF2 rabbit polyclonal antibody. (TA590235)
Immunohistochemical staining of human cerebellum shows distinct cytoplasmic positivity in Purkinje cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.