Home Antibody All anti-TYRO3 antibodies
Anti-TYRO3 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for TYRO3 (NM_006293)
|DNA immunization. This antibody is specific for the N Terminus Region of the target protein.|
|Human , Dog , Rat , Monkey , Mouse
|| WB 1:5000~20000,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens TYRO3 protein tyrosine kinase (TYRO3)|
|BYK; Dtk; RSE; Sky; Tif|
|Tyrosine-protein kinase (TYRO3) belongs to the Tyr protein kinase family (AXL/UFO subfamily). The UFO family of receptor tyrosine kinases is comprised of subfamily members Rse(also referred to as Tyro3 or Sky) and UFO (also called Tyro7 or Axl). Two distinct isoforms of Rse, designated Brt and Etk-2, have been described. Brt differs from Rse at its C-terminus, but more importantly lacks the N-terminal 31 amino acid signal peptide sequence present in Rse, which is replaced by a 27 amino acid Brt-specific sequence. It has been suggested that as a result of this alternative splicing event, Brt resides in the cytoplasm, unlike Rse which is expressed on the cell surface. Ekt-2 also lacks an N-terminal signal peptide which is substituted with a 45 amino acid Ekt-2-specific sequence. Protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (RC208260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TYRO3 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).