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Home Antibody All anti-AKT1 antibodies
UltraMAB

Anti-AKT1 GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA590196
  • Rabbit Polyclonal AKT1 Antibody
100ug 325 In Stock
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IHC(15)
IF(1)
ELISA(1)
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Also for AKT1 (NM_005163)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the C Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration1mg/ml
Guaranteed Application *WB, ELISA, IHC, IF Suggested Dilutions WB 1:5000~20000, IHC 1:150, IF 1:500,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 1
Alternative NameAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Database LinkNP_005154
Entrez Gene 207 Human
Entrez Gene 11651 Mouse
Entrez Gene 24185 Rat
Entrez Gene 697747 Monkey
Entrez Gene 490878 Dog
FunctionAKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (RC220257, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AKT1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
ELISA Image
ELISA: AKT1 Antibody
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IF Image
Immunofluorescent staining of HeLa cells using anti-AKT1 rabbit polyclonal antibody (TA590196).

 

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