Home Antibody All anti-BRAF antibodies
Anti-BRAF GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for BRAF (NM_004333)
|DNA immunization. This antibody is specific for the Middle Region of the target protein.|
|Human , Dog , Rat , Monkey , Mouse
|| WB 1:5000~20000,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF)|
|B-RAF1; BRAF1; NS7; RAFB1|
|B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.|
MAPK signaling pathway
Senescence and Autophagy
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BRAF polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).