Home Antibody All anti-SOX2 antibodies
Anti-SOX2 GAT Antibody Polyclonal
UltraMAB Antibodies - Validated with 10K Protein Chip
Also for SOX2 (NM_003106)
|DNA immunization. This antibody is specific for the Middle Region of the target protein.|
|WB, ELISA, DB
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody. (Protein A or G Sepharose)
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens SRY (sex determining region Y)-box 2 (SOX2)|
|SRY (sex determining region Y)-box 2. Entrez Protein NP_003097. It is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).|
Wnt Signaling Pathway
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Western Blot: SOX2 Antibody - Cell lysate from 293 over expressing Sox2 was subjected to SDS-PAGE and transfer to nitrocellulose membrane. The membrane was incubated with anti-Sox2 antibody (1:1000 dilution) and then probed anti-rabbit IgG-HRP. Signals were acquired with a CCD camera. Arrow points to Sox2. Data courtesy of a SDI customer.
Dot Blot: SOX2 Antibody - The anti-Sox2 antibody (right quadruplicate spots at each panel) or anti-c-Jun antibody (left quadruplicate spots at each panel) were printed on membranes at 10 ng/spot. The membranes were incubated with biotinylated cell lysate (50 ug per membrane), followed by probing with streptavidin-HRP and CCD camera image acquisition. (a), Cell lysate from undifferentiated mouse embryonic stem cells; (b), Cell lysate from differentiated mouse embryonic stem cells. Data courtesy of a SDI customer.
Dot Blot: SOX2 Antibody - Protein samples (upper left quadruplicate spots: bovine gamma globulin; upper right quadruplicate spots: cell lysate from 293 cell over expressing Nanog; below left quadruplicate spots: cell lysate from 293 cell over expressing Sox2; below right quadruplicate spots: cell lysate from 293 cell over expressing POU5F1) were printed on membranes at 10 ng/spot. The membrane was incubated with anti-Sox2 antibody (1:1000 dilution) and then probed anti-rabbit IgG-HRP. Signals were acquired with a CCD camera. Data courtesy of a SDI customer.