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Home Antibody All anti-SOX2 antibodies

Anti-SOX2 GAT Antibody Polyclonal

Generated by Genomic Immunization


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA590150 Rabbit Polyclonal SOX2 Antibody 100ug $325 2 Weeks
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OriGene Data

ImmunogenDNA immunization. This antibody is specific for the Middle Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Mouse Concentration0.72482 mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens SRY (sex determining region Y)-box 2 (SOX2)
Alternative NameANOP3; MCOPS3
Database LinkNP_003097
Entrez Gene 6657 Human
Entrez Gene 20674 Mouse
FunctionSRY (sex determining region Y)-box 2. Entrez Protein NP_003097. It is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
Related PathwayES Cell Differentiation/IPSAdult stem cellsCancer stem cellsEmbryonic stem cellsInduced pluripotent stem cellsTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX2 (RC200757, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX2.

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SOX2 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

WB Image
Western Blot: SOX2 Antibody - Cell lysate from 293 over expressing Sox2 was subjected to SDS-PAGE and transfer to nitrocellulose membrane. The membrane was incubated with anti-Sox2 antibody (1:1000 dilution) and then probed anti-rabbit IgG-HRP. Signals were acquired with a CCD camera. Arrow points to Sox2. Data courtesy of a SDI customer.

ELISA: SOX2 Antibody

IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SOX2 rabbit polyclonal antibody. (TA590150)

IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-SOX2 rabbit polyclonal antibody. (TA590150)


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