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Home Antibody All anti-PCNA antibodies
UltraMAB

Anti-PCNA GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA590137
  • Rabbit Polyclonal PCNA Antibody
100ug $325 In Stock
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WB(2)
IHC(10)
ELISA(1)

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the Middle Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration0.9mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:5000~20000, IHC 1:150, ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens proliferating cell nuclear antigen (PCNA), transcript variant 1
Alternative NameATLD2
Database LinkNP_002583
Entrez Gene 5111 Human
Entrez Gene 18538 Mouse
Entrez Gene 25737 Rat
Entrez Gene 718006 Monkey
Entrez Gene 477166 Dog
FunctionPCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Related PathwayStem cell - PluripotencyDruggable Genome DNA replicationBase excision repairNucleotide excision repairMismatch repairCell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA (RC201741, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCNA.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PCNA polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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ELISA: PCNA Antibody
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PCNA Rabbit Polyclonal antibody. (TA590137)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PCNA Rabbit Polyclonal antibody. (TA590137)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PCNA Rabbit Polyclonal antibody. (TA590137)
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Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PCNA Rabbit Polyclonal antibody. (TA590137)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PCNA Rabbit Polyclonal antibody. (TA590137)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PCNA Rabbit Polyclonal antibody. (TA590137)
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Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-PCNA Rabbit Polyclonal antibody. (TA590137)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PCNA Rabbit Polyclonal antibody. (TA590137)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-PCNA Rabbit Polyclonal antibody. (TA590137)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-PCNA Rabbit Polyclonal antibody. (TA590137)
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