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Anti-CCNE1 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for CCNE1 (NM_001238)
|DNA immunization. This antibody is specific for the N Terminus Region of the target protein.|
|WB, ELISA, IHC
|| WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens cyclin E1 (CCNE1)|
|Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.|
TGF Beta Signaling Pathway
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCNE1 (RC204289, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCNE1.
Western Blot: Cyclin E Antibody - Western Blot was performed using affinity purified SEQer CCNE1 antibody, aa(7-106) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer CCNE1 antibody, aa(7-106) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CCNE1 Rabbit Polyclonal antibody. (TA590076)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-CCNE1 Rabbit Polyclonal antibody. (TA590076)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CCNE1 Rabbit Polyclonal antibody. (TA590076)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CCNE1 Rabbit Polyclonal antibody. (TA590076)