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Home Antibody All anti-CCNE1 antibodies
UltraMAB

Anti-CCNE1 GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA590076
  • Rabbit Polyclonal Cyclin E Antibody
100ug 325 In Stock
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IHC(4)
ELISA(1)

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the N Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman Concentration1.01mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens cyclin E1 (CCNE1)
Alternative NameCCNE
Database LinkNP_001229
Entrez Gene 898 Human
FunctionCyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Related PathwayStem cell relevant signaling - DSL/Notch pathwayStem cell - PluripotencyTranscription FactorsDruggable Genome Cell cycleOocyte meiosisp53 signaling pathwayPathways in cancerProstate cancerSmall cell lung cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCNE1 (RC204289, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCNE1.
WB Image
Western Blot: Cyclin E Antibody - Western Blot was performed using affinity purified SEQer CCNE1 antibody, aa(7-106) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer CCNE1 antibody, aa(7-106) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
ELISA Image
ELISA: Cyclin E Antibody
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CCNE1 Rabbit Polyclonal antibody. (TA590076)
IHC Image
Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-CCNE1 Rabbit Polyclonal antibody. (TA590076)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CCNE1 Rabbit Polyclonal antibody. (TA590076)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CCNE1 Rabbit Polyclonal antibody. (TA590076)

 

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