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Anti-ANGPT1 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for ANGPT1 (NM_001146)
|DNA immunization. This antibody is specific for the Middle Region of the target protein.|
|Human , Dog , Monkey
|WB, ELISA, IF
|| WB 1:5000, IF 1:500,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens angiopoietin 1 (ANGPT1), transcript variant 1|
|AGP1; AGPT; ANG1|
Entrez Gene 284 Human
Entrez Gene 697017 Monkey
Entrez Gene 403656 Dog
|Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver.To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody gives a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.|
|Secreted ProteinES Cell Differentiation/IPSDruggable Genome |
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANGPT1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunofluorescent staining of HeLa cells using anti-ANGPT1 rabbit polyclonal antibody (TA590068).