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Anti-ALPL GAT Antibody Polyclonal
Generated by Genomic Immunization
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Also for ALPL (NM_000478)
|DNA immunization. This antibody is specific for the Middle Region of the target protein.|
|Human , Mouse
|WB, ELISA, IHC, IF
||WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 1|
|AP-TNAP; APTNAP; HOPS; TNAP; TNSALP|
Entrez Gene 249 Human
Entrez Gene 11647 Mouse
|There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.|
|Druggable Genome Folate biosynthesisMetabolic pathways|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALPL (RC205692, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALPL.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ALPL polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
ELISA: Alkaline Phosphatase, Tissue Non-Specific Antibody
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ALPL rabbit polyclonal antibody. (TA590021)
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic and membranous positivity in cortical cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.