Home Antibody All anti-ALDOB antibodies
Anti-ALDOB GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for ALDOB (NM_000035)
|DNA immunization. This antibody is specific for the N Terminus Region of the target protein.|
|Human , Dog , Rat , Monkey , Mouse
|ELISA, WB, IHC, IF
||WB 1:5000~20000,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens aldolase, fructose-bisphosphate B (ALDOB)|
Entrez Gene 229 Human
Entrez Gene 230163 Mouse
Entrez Gene 24190 Rat
Entrez Gene 713818 Monkey
Entrez Gene 474787 Dog
|Fructose-1,6-bisphosphate aldolase (EC 188.8.131.52 ) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.|
|Druggable Genome Glycolysis / GluconeogenesisPentose phosphate pathwayFructose and mannose metabolismMetabolic pathways|
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ELISA: Aldolase B Antibody
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ALDOB polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDOB (RC220062, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDOB.
Immunohistochemical staining of human kidney shows strong positivity in proximal tubules.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-2 OS shows no positivity.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.