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Anti-GCLC TRUEMAB Antibody Clone 1A3
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for GCLC (NM_001498)
|Full length human recombinant protein of human GCLC(NP_001489) produced in HEK293T cell.|
|Human , Rat , Monkey
|| WB 1:200~1000, IHC 1:150,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant 1|
|GCL; GCS; GLCL; GLCLC|
Entrez Gene 2729 Human
Entrez Gene 25283 Rat
Entrez Gene 710508 Monkey
|Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct |
|Druggable Genome Glutathione metabolismMetabolic pathways|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCLC (RC207915, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCLC.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GCLC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-GCLC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507318)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GCLC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507318)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-GCLC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507318)