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Home Antibody All anti-HPRT1 antibodies

Anti-HPRT1 TRUEMAB Antibody Clone OTI2D5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA506987 HPRT1 mouse monoclonal antibody, clone OTI2D5 (formerly 2D5) 100ul $325 In Stock
LC400070 HPRT1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF506987 Carrier-free (BSA/glycerol-free) HPRT1 mouse monoclonal antibody, clone OTI2D5 (formerly 2D5) 100ug $450 8-10 Days
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WB(1)
IF(1)
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Also for HPRT1 (NM_000194)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human HPRT1(NP_000185) produced in HEK293T cell.
Clone NameClone OTI2D5 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IF Suggested DilutionsWB 1:2000, IF 1:100
Predicted MW Explanation 24.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens hypoxanthine phosphoribosyltransferase 1 (HPRT1)
Alternative NameHGPRT; HPRT
Database LinkNP_000185
Entrez Gene 3251 Human
FunctionThe protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009].
Related PathwayStem cell - PluripotencyDruggable Genome Purine metabolismDrug metabolism - other enzymesMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HPRT1 (RC200462, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HPRT1.
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Immunofluorescent staining of HeLa cells using anti-HPRT1 mouse monoclonal antibody (TA506987) at 1:100 dilution.
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